Coding

Part:BBa_K2868020:Design

Designed by: Cale Lester   Group: iGEM18_Stanford-Brown-RISD   (2018-10-09)


mfp151: Recombinant mussel foot protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because of the repeated motifs it was difficult to synthesize the DNA using IDT gBlocks. By splitting the gene into two orders and then using gibson assembly to put them back together, this can be overcome.


Source

The amino acid sequence posted by the Greensborough-Austin 2013 iGEM team (here: http://2013.igem.org/Team:Greensboro-Austin/MAPs) was reverse translated into DNA using online IDT sequence tools, and was then codon optimized for use in E.Coli.

References